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The combination of HPLC-MS is oriented to the precise detection and possible identification of chemicals from the presence of other chemical compounds. On the other hand, it is hard to interface the liquid chromatography to the mass-spectrometer, simply because each of the solvents need to be eliminated very first. The popular used interface involv
With this sort of stationary phases, retention time is extended for lipophylic molecules, Whilst polar molecules elute a lot more quickly (arise early during the analysis). A chromatographer can raise retention occasions by incorporating extra water to the mobile stage, therefore creating the interactions from the hydrophobic analyte While using th